Coding

Part:BBa_K497002

Designed by: Joanna Hare   Group: iGEM10_Macquarie_Australia   (2010-10-24)

Agrobacterium tumefaciens bacteriophytochrome with 3

The Agrobacterium tumefaciens bacteriophytochrome binds to biliverdin, a chromophore. Biliverdin is produced in the heme metabolic pathway catalysed by heme oxygenase. Once bound to the chromophore the complex can absorb red and far red light causing the complex to change colour from blue to green reversibly. There is a 3' transcriptional terminator. Sequence from National Center for Biotechnology Information .


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1652
    Illegal PstI site found at 2048
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1652
    Illegal PstI site found at 2048
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1855
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1652
    Illegal PstI site found at 2048
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1652
    Illegal PstI site found at 2048
    Illegal NgoMIV site found at 1184
    Illegal NgoMIV site found at 1682
    Illegal AgeI site found at 1747
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None